The quantitative method is based on a real-time PCR (Perkin-Elmer Applied Biosystems, ABI Prism 7700), with allele-specific primers for DNA sequences containing single nucleotide polymorphisms (SNPs) ...

Nagoya, Japan - Polymerase chain reaction (PCR) is a simple and ubiquitous method in molecular biology for amplifying DNA segments into millions of copies. This is important not only for basic ...

A 1.4-Mb tandem duplication, including the gene for peripheral myelin protein 22 (PMP22) in chromosome 17p11.2-12 is responsible for 70% of the cases of the demyelinating type 1 of Charcot-Marie-Tooth ...

Standard PCR systems require users to set a predefined number of cycles relative to the assay and the input amount. This requires quantifying input material and splitting samples of varying inputs ...

Sample volumes that are gradually increasing and innovative options for molecular biology experiments are altering the demands on quantitative real-time PCR. Migrating to the multi-well format with ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Study Shows How Melting Point Analysis Infers the Presence of and Identifies SNPs The melting temperature (T m) of a PCR product is dependent on both the length and sequence of the DNA. Melting curve ...

Optimizing the formulation of reagents for your qPCR assay requires careful experimental design that looks across several performance factors for each reagent formulation tested. These performance ...

Promega Corporation has announced that it has launched GoTaq® Probe PCR Master Mix, GoTaq Probe 1-Step RT-qPCR System, and GoTaq Probe 2-Step RT-qPCR System. These ready-to-use, 2x master mixes ...