Understand the stages of a standard RNA-seq bioinformatics workflow, including the role of QC, alignment, gene expression quantification, and specialized application analyses Identify key QC metrics ...

Since the first human genome was sequenced in 2000, omic profiling technologies have seen their costs reduced by multiple orders of magnitude, and omic profiling is now performed routinely. Petabytes ...

Intervertebral disc degeneration (IDD) is marked by nucleus pulposus (NP) fibrosis, but its molecular drivers remain unclear. This study investigated NP cellular heterogeneity and key regulators using ...

Transcriptome-wide profiling of bacterial RNA pseudouridylation maps modification landscapes and nominates candidate regulatory sites, offering a resource and foundation for future mechanistic studies ...