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API (Analytical Profile Index). There's different types of API techniques (API 20E (Enterobacteriaceae), API 20NE (non enterobacteriaceae), API 20 Staph. 20 referee to 20 biochemical tests. Before working API techniques, you must perform the following. 1-fresh cultures must be prepared (young colonies 18-24hr incubation) 2- Gram stain testing. 3- Oxidase test to differentiate between the positive Oxidase Pseudomonas aeruginosa and oxidase negative Enterobacteriaceae. 4 - API is used as a confirm
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