50 µL plasma sample (or calibration standard or QC sample) was added with 25 µL IS working solution and 200 µL cold methanol. After vortexing for 3 min and centrifuging at 10000 rpm for 5 min, 100 µL ...
High-performance liquid chromatography (HPLC) or ultra-high-performance liquid chromatography (UHPLC) serves as the front end of the analysis, reducing sample complexity before mass detection.
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