Light that is reflected back from the edge of the medium it is traveling through. When light rays travel at an angle greater than the "critical" angle, which is determined by the medium, the light ...
In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...
Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from ...
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