The answer depends on the questions you’re trying to answer, as well as the origin, quantity, and quality of your samples. In this article, we’ll briefly discuss several RNA-seq workflows, how they ...

cDNA is produced from RNA templates via reverse transcription and is used in applications like RT-qPCR, microarrays, and RNA-seq. High-quality synthesis depends on intact RNA, removal of genomic DNA, ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...

Samples are commonly stored in a way that degrades RNA. Scientists are devising new ways to overcome this obstacle for RNA sequencing. RNA is more subject to degradation than DNA, whether chemical, ...

Genes are expressed when messenger RNA is made from DNA, which is then translated into proteins. Researchers use single-cell and bulk RNA-seq analysis to study gene expression, creating vast amounts ...